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Image Search Results
Journal: bioRxiv
Article Title: Experimental tests of functional molecular regeneration via a standard framework for coordinating synthetic cell building
doi: 10.1101/2021.03.03.433818
Figure Lengend Snippet: SDS-PAGE results for each custom dropout Solution B (NEB). (a) from left to right, normal Solution B (full), missing all tRNA synthetases (△aaRSs), missing T7 RNA polymerase (△T7 RNAP), missing all release factors (△RFs), and missing all initiation factors (△IFs). (b) from left to right, normal Solution B (full), missing ribosome and tRNA recycling factors (△RRF/MTF), missing elongation factors (△EFs), and missing energy recycling factors (△Ks). A broad range pre-stained protein standard (NEB #P7712) was used as a ladder for each gel. Expected location of specific components as noted (red, orange).
Article Snippet: To construct the remaining 16 single-enzyme depletion PURE variants we sourced six different
Techniques: SDS Page, Staining